Aptamers for the diagnosis of lung cancer
Objective: to obtain DNA aptamers for the detection of lung cancer biomarkers in blood and tissue
Aptamers were selected from a DNA library of oligonucleotides using the Cell SELEX method. Postoperative tissues of patients with non-small cell lung cancer were used as a positive target for selection; adjacent tissues and whole blood of healthy people were used as a negative target.
Aptamers that specifically bind to lung cancer cells and proteins have been obtained through selection (www.sciencedirect.com). Using mass spectrometry studies, putative aptamer target proteins such as neutrophil defensin 1 and 3, vimentin, actin were identified (www.sciencedirect.com). Comparative analysis of histological sections of lung cancer tissues, adjacent tissues and lungs of healthy people showed that the obtained aptamers stain the membranes and nuclei of malignant cells and transformed vessels (www.sciencedirect.com). It was shown that the obtained aptamers can be used to detect circulating tumor cells and microemboli (www.sciencedirect.com), as well as proteins (www.nature.com, www.sciencedirect.com) in the blood and plasma of unhealthy people.
Aptamers for intraoperative staining of brain glioblastoma
Objective: to obtain DNA aptamers capable of specifically contrasting the boundaries of brain glioblastoma in vivo
Aptamers were selected from a DNA library of oligonucleotides using the Cell SELEX method to postoperative glioblastoma tissues. Tissues of benign brain tumors and conventionally healthy brain tissues adjacent to tumor ones were used as negative targets.
Aptamers for the detection of bacteria Salmonella typhimurium and Salmonella eneritidis
Objective: To obtain aptamers for the detection and determining the type of Salmonella typhimurium and Salmonella eneritidis
Highly specific DNA aptamers to live Salmonella typhimurium and Salmonella eneritidis were selected via the SELEX technique.
Twelve rounds of selection were performed; each comprised a positive selection step against viable S. typhimurium or S. eneritidis and a negative selection step against heat killed S. typhimurium or S. eneritidis and a mixture of related pathogens, including Salmonella enteritidis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Citrobacter freundii to ensure the species specificity of the selected aptamers.