Aptamers for the diagnosis of lung cancer
Objective: to obtain DNA aptamers for the detection of lung cancer biomarkers in blood and tissue
Aptamers were selected from a DNA library of oligonucleotides using the Cell SELEX method. Postoperative tissues of patients with non-small cell lung cancer were used as a positive target for selection; adjacent tissues and whole blood of healthy people were used as a negative target.
Aptamers that specifically bind to lung cancer cells and proteins have been obtained through selection (www.sciencedirect.com). Using mass spectrometry studies, putative aptamer target proteins such as neutrophil defensin 1 and 3, vimentin, actin were identified (www.sciencedirect.com). Comparative analysis of histological sections of lung cancer tissues, adjacent tissues and lungs of healthy people showed that the obtained aptamers stain the membranes and nuclei of malignant cells and transformed vessels (www.sciencedirect.com). It was shown that the obtained aptamers can be used to detect circulating tumor cells and microemboli (www.sciencedirect.com), as well as proteins (www.nature.com, www.sciencedirect.com) in the blood and plasma of unhealthy people.