We Solve medical tasks using working aptamers

Aptamer Selection, Characterization, 3D Structure Simulation And Target Identification

IDENTIFICATION OF PROTEIN TARGETS

We will perform identification and verification of yours aptamers targets, just click the button

identification and verification of probable protein targets are needed

IF YOU DO NOT KNOW THE APTAMER TARGET AND NEED TO IDENTIFY IT

Identification of the most probable protein targets of the aptamer is performed using mass spectrometry. AptaBiD technology is used to isolate target proteins from the cell mixture. An array of these proteins obtained after mass spectrometric studies is processed, after which a list of the most likely targets is formed.

IF YOU KNOW THE APTAMER TARGET AND NEED TO VERIFY IT

We offer indirect methods for target verification using antibodies specific to the intended target. The first method is based on the competitive replacement of aptamers by appropriate antibodies from the cell surface. And the second method allows you to visualize areas of joint localization of aptamers with antibodies in histological sections.

STAGES OF IDENTIFICATION AND VERIFICATION of targets

1

stage

Isolation of proteins specific to aptamers from the sample containing cells or minced tissue using AptaBiD technology.

2

STAGE

Identification of proteins in the sample by mass spectrometry.

3

STAGE

Analysis of mass spectrometric data and identification of the probable protein targets.

4

STAGE

Verification of the probable target with the method of competitive replacement of aptamers by antibodies using flow cytometry.

5

STAGE

Verification of the probable target using the method of colocolization of aptamers and corresponding antibodies by laser scanning microscopy.

WE USE INNOVATIve TECHNOLOGIES

APTABID

AptaBiD allows us to isolate proteins that bind to specific aptamers. With the help of this method it becomes possible to determine the direct target of the aptamer.

TISSUE SELEX

The method for generating aptamers which are capable to bind with tissue targets.

CELL SELEX

This technology involves the selection of aptamers for the whole cells. This allows us to obtain aptamers that are able to bind to the target in its native conformation.

LIGS

The method identifies highly selective aptamers against a predetermined epitope expressed on the cell surface. Monoclonal antibody interacts with its cognate epitope to outcompete and replace specific aptamers from an enriched SELEX pool.

SAXS

This method is used for determination of the nanoscale density differences in the sample. This makes it possible to determine the size and shape of the macromolecule.

HT SELEX

HT SELEX is the method that increases the sensitivity over traditional cloning and sequencing applications and reduces the number of rounds required to achieve detectable enrichment.

THE RESULTS YOU'LL GET

Information about the most probable aptamer's target

If a new aptamer was selected using the cell-SELEX or tissue-SELEX methods, the molecular targets to which the aptamer binds on the cell surface may not be known in advance. To determine protein targets of aptamers, an affinity enrichment method is used, followed by identification of the target candidate proteins by mass spectrometry.

Mass spectrometry data

As a result of mass spectrometric analysis of the enriched protein mixture obtained after incubation with the aptamer, a list of candidate proteins for the aptamer target is obtained. After comparison with controls and discarding of non-membrane and non-specific proteins, this list is reduced to 4-5 proteins, the most probable target candidates.

SCIENTISTS INVOLVED IN THE STUDY

Changing your mind and changing world
CHIEF RESEARCHER

YURIJ GLAZYRIN

Ph.D. in Biological Sciences
Changing your mind and changing world
LEADING RESEARCHER

ANNA BLAGODATOVA

Ph.D. in Biological Sciences
Changing your mind and changing world
LEADING RESEARCHER

OLGA KOLOVSKAYA

Ph.D. in Biological Sciences
Changing your mind and changing world
LEADING RESEARCHER

GALINA ZAMAY

Ph.D. in Biological Sciences

ORDER TARGET IDENTIFICATION

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