Aptamer Selection, Characterization, 3D Structure Simulation And Target Identification
IDENTIFICATION OF PROTEIN TARGETS
Identification of the most probable protein targets of the aptamer is performed using mass spectrometry. AptaBiD technology is used to isolate target proteins from the cell mixture. An array of these proteins obtained after mass spectrometric studies is processed, after which a list of the most likely targets is formed.
We offer indirect methods for target verification using antibodies specific to the intended target. The first method is based on the competitive replacement of aptamers by appropriate antibodies from the cell surface. And the second method allows you to visualize areas of joint localization of aptamers with antibodies in histological sections.
Isolation of proteins specific to aptamers from the sample containing cells or minced tissue using AptaBiD technology.
Identification of proteins in the sample by mass spectrometry.
Analysis of mass spectrometric data and identification of the probable protein targets.
Verification of the probable target with the method of competitive replacement of aptamers by antibodies using flow cytometry.
Verification of the probable target using the method of colocolization of aptamers and corresponding antibodies by laser scanning microscopy.