We Solve medical tasks using working aptamers

Aptamer Selection, Characterization, 3D Structure Simulation And Target Identification

APTAMER CHARACTERIZATION

We will perform characterization of your aptamers, just click the button

APTAMER CHARACTERIZATION IS NEEDED

IF YOU WANT TO ESTIMATE THE AFFINITY AND SPECIFICITY OF THE APTAMER

After mathematical analysis of the sequences obtained by Next Generation Sequencing, up to 10 aptamers are selected, which are then synthesized chemically. Their specificity and affinity for the target is assessed using flow cytometry and microscopy. The experiments on the binding of aptamers to positive and negative targets are performed in several replicates and are statistically processed. As a result of the analysis, 1-3 aptamers with the best characteristics are selected.

IF YOU WANT TO ESTIMATE THE DISSOCIATION CONSTANT OF THE APTAMER

The dissociation constant (Kd) allows one to evaluate at what concentrations the target and aptamer enter the equilibrium state. This value allows one to indirectly assess the optimal concentration of aptamers for binding. Experiments to evaluate Kd of aptamers and a positive target are performed by flow cytometry.

STAGES OF APTAMER CHARACTERIZATION

1

stage

Aptamers affinity for target tissues, cells, bacteria or fungi is estimated using flow cytometry.

2

STAGE

Aptamers specificity for negative tissues, cells, bacteria or fungi is estimated using flow cytometry and laser scanning microscopy.

3

STAGE

The dissociation constant (Kd) of an aptamer and it's target is estimated using flow cytometry.

WE USE INNOVATIve TECHNOLOGIES

APTABID

AptaBiD allows us to isolate proteins that bind to specific aptamers. With the help of this method it becomes possible to determine the direct target of the aptamer.

TISSUE SELEX

The method for generating aptamers which are capable to bind with tissue targets.

CELL SELEX

This technology involves the selection of aptamers for the whole cells. This allows us to obtain aptamers that are able to bind to the target in its native conformation.

LIGS

The method identifies highly selective aptamers against a predetermined epitope expressed on the cell surface. Monoclonal antibody interacts with its cognate epitope to outcompete and replace specific aptamers from an enriched SELEX pool.

SAXS

This method is used for determination of the nanoscale density differences in the sample. This makes it possible to determine the size and shape of the macromolecule.

HT SELEX

HT SELEX is the method that increases the sensitivity over traditional cloning and sequencing applications and reduces the number of rounds required to achieve detectable enrichment.

THE RESULTS YOU'LL GET

Information about aptamer affinity and specificity

Affinity related to the strength of interaction that may exist between an aptamer and it's target. Usually aptamers that have slow-off rates show strong interactions with their target. These high affinity aptamers can bind low amounts of target making them sensitive reagents. Also in the development of any diagnostic tests, it is important that aptamer would recognize only the desired target and would not cross-react with any other non-target molecule presented in the sample mixture. This selective binding of aptamers to the chosen target molecule and no other in the entire sample mixture is defined as the specificity. We could provide you information about aptamers with most affinity and specifity to your target.

FLUORESCENCE POLARIZATION

Fluorescence polarization assay could be used to estimate an interaction between aptamers and small molecule target such as peptides, proteins. This approach is based on the induced-fit binding mechanism of nucleic acid aptamers which was used to transform the small target binding event into a detectable fluorescence anisotropy signal.

Laser Scanning Microscopy data

Laser scanning microscopy data could be used for visual illustration of aptamer binding with its target.

FLOW CYTOMETRY DATA

Flow cytometry facilitates quantitative, multi-parameter analysis of target cell populations (protein, virus, etc) based on the binding of target with the ligand. This method is used to get information about aptamer specifity and affinity.

SCIENTISTS INVOLVED IN THE STUDY

Changing your mind and changing world
CHIEF RESEARCHER

ANNA KICHKAILO

Ph.D. in Biological Sciences, Assistant Professor
Changing your mind and changing world
LEADING RESEARCHER

ANNA BLAGODATOVA

Ph.D. in Biological Sciences
Changing your mind and changing world
LEADING RESEARCHER

OLGA KOLOVSKAYA

Ph.D. in Biological Sciences
Changing your mind and changing world
LEADING RESEARCHER

GALINA ZAMAY

Ph.D. in Biological Sciences

ORDER APTAMER CHARACTERIZATION

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