APTAMERS FOR THE DETECTION OF BACTERIA SALMONELLA TYPHIMURIUM AND SALMONELLA ENERITIDIS

HIGH SPECIFICITY

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We develop tools for diagnostics and treatment of socially significant diseases based on DNA-Aptamers.

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Aptamers for the detection of bacteria Salmonella typhimurium and Salmonella eneritidis

Objective: To obtain aptamers for the detection and determining the type of Salmonella typhimurium and Salmonella eneritidis

Highly specific DNA aptamers to live Salmonella typhimurium and Salmonella eneritidis were selected via the SELEX technique.

Twelve rounds of selection were performed; each comprised a positive selection step against viable S. typhimurium or S. eneritidis and a negative selection step against heat killed S. typhimurium or S. eneritidis and a mixture of related pathogens, including Salmonella enteritidis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Citrobacter freundii to ensure the species specificity of the selected aptamers.

Based on the obtained aptamers, electrochemical sensors were made to detect salmonella (pubs.acs.org) and determine their type (pubs.acs.org). A method for determining the antibiotic resistance of salmonella S. enteritidis and S. typhimurium using DNA aptamers was developed, which is based on the ability of aptamers to bind only to living bacteria (Patent RU 2518372). It was found that a mixture of synthetic DNA aptamers to S. enteritidis and S. typhimurium inhibits the growth of bacterial colonies by more than 70% (pubs.acs.org).

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